Modulation of Epigenetics research
The aims of the Modulation of Epigenetics proposed research are:
1. This study is aimed at validating a genetic tool to manipulate the specific methylation of mitochondrial DNA polymerase (pol g) gene by an epigenetic regulation.
2. The sequences of DNA will be cloned and validation specific sequences for the Polymerase gamma (POLG).
3. To perform tissue culture transfection of mammalian cells with dCAS9- methylase plasmid system.
4. To isolate DNA and assess the specific gene methylations by qPCR amplification.
5. To validate methylation studies by protein expression analysis (e.g. by western blot).
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